A huge treasure trove of ancient DNA from animals including extinct woolly mammoths has been discovered in frozen squirrel feces in Canada’s remote Yukon territory, scientists said Tuesday.

The DNA found deep inside sealed-off burrows is between 3,000 and 700,000 years old, offering a rare window into how life has changed over the millennia.

As well as DNA from woolly mammoths – which the US company Colossal claims it is trying to “de-extinct” – genetic material was also found from wolves, bison, horses, a cheetah and hundreds of plants.

Tyler Murchie, a paleogenomics researcher at Canada’s McMaster University and lead author of a new study, admitted that digging through squirrel poop might sound “less appealing” than discovering, say, a mammoth tusk.

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However, the “spectacular” amount of information they uncovered suggests that feces is an overlooked way to see into our planet’s distant past, he added.

The scientists had just been expecting to study the squirrel’s microbiome before coming across the “really surprising biodiversity of organisms”, Murchie said.

It turned out that arctic ground squirrels were ideal subjects for this research because of their “natural archivist behavior”, he explained.

The squirrels are only conscious for around four months a year, spending the rest of their life in hibernation.

An arctic ground squirrel stands upright among sunlit grass and low shrubs, looking alert in a tundra-like landscape.
Researchers analyzed permafrost samples collected from ground squirrel burrows that span several glacial periods and can remain frozen and sealed for thousands of years. (Government of Yukon)

So when they are awake, “they’ve got to get out there and eat as much as they can of everything,” Murchie said.

The squirrels pack their burrows with nuts, seeds, leaves, bones, fur and anything else they can find.

But over time, rising permafrost permanently sealed off some of the burrows in the Yukon, creating a perfectly preserved time capsule.

Murchie said they even found a “super cute little guy” frozen in time.

“He just went to sleep one season, then he never woke up… it wasn’t until some paleontologist came by investigating that they found him in there.”

Composite image showing an excavated section of pale sediment at the Gold Run site, with a shovel for scale, a close-up of a labeled nest latrine, and dark fossilized fecal samples prepared for ancient DNA subsampling.
Photo taken during field recovery from the Gold Run site in Canada’s remote Yukon territory. Insets show a nest latrine and samples. (Murchie et al., Nat. Commun., 2026)

Back from the dead?

The scientists used the DNA to reconstruct 18 mitochondrial genomes, including for six woolly mammoths that lived in different eras.

This involves using computers to stitch together DNA fragments, like puzzle pieces, Murchie explained.

Colossal has declared its intention to resurrect the woolly mammoth, which went extinct around 4,000 years ago.

However, experts have expressed skepticism about the claim, saying the resulting animal would be more like an Asian elephant with some genetic tweaks to make it resemble a mammoth.

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Murchie, who does not work for Colossal, said the genetic data they found would be made publicly available, so the company could use it.

“But they already have so much DNA to go off of – whole genomes from different organisms – so I’m sure ours is a drop in the bucket,” he added.

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The team behind the latest research, which was published in the journal Nature Communications, is working on another study describing what the DNA reveals about the woolly mammoth’s evolution.

Murchie could not speak about that future research, other than to say it was “super cool”.

“I can’t believe that we were able to get these insights from squirrel feces,” he added.

© Agence France-Presse